TSKgel FcR-ⅢA-5PW Preparative Affinity Column, 5 mg Antibody Load (CAT#: STEM-BC-3233-ZJF)

Highlights

Preparative Fc-receptor affinity

5 mg sample loading

Glycan-dependent selectivity

Cat Number: STEM-BC-3233-ZJF

Application: for preparative separation of antibody activity variants

Model: TSKgel FcR-ⅢA-5PW




Description

This preparative affinity column is designed to isolate antibody fractions according to Fc glycan-dependent receptor interaction. It uses the same recombinant human FcγRⅢA ligand as the analytical FcR-ⅢA-NPR column. This provides equivalent separation selectivity in a larger preparative format. The column can process up to 5 mg of antibody in a single run. It supports similarity assessment between biosimilar and reference antibodies. It can also be used for cell-line screening and culture-condition optimization. Prepared fractions may be used for glycan characterization and biological-activity testing. The PEEK column body and hydrophilic nonporous matrix support low nonspecific adsorption.

Specification

Chromatography mode: Fc-receptor affinity
Matrix: Hydrophilic nonporous polymer
Ligand: Recombinant human FcγRⅢA
Particle size: 10 µm
Column dimensions: 7.8 mm I.D. × 7.5 cm
Column material: PEEK
Maximum antibody loading: 5 mg
Catalog number: 23532
Applications: Biosimilar comparison, cell-line screening, culture optimization, process monitoring, batch control, and antibody-fraction preparation

Features

Preparative Fc-receptor affinity
5 mg sample loading
Glycan-dependent selectivity
ADCC-related fractionation
PEEK low-binding hardware
Cell-line screening support
Process-monitoring capability
Suitable for activity studies

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