Transfer Buffer, 10X, 1L (CAT#: STEM-GEN-0163-LC)

10X Transfer Buffer is a concentrated solution commonly used for electroblotting proteins from SDS-PAGE gels to membranes (such as PVDF or nitrocellulose) during Western blotting. It contains optimized concentrations of Tris, and glycine, which maintain proper pH and conductivity to ensure efficient protein transfer under an electric field. This buffer enables consistent and reliable migration of proteins from the gel to the membrane while minimizing diffusion and maintaining transfer efficiency.

pH: 8.3
Tris: 25 mM
Glycine: 192 mM
Sterile-filtered