TOYOPEARL Phenyl FT-750F Flow-Through HIC Medium,>100 nm Pores (CAT#: STEM-BC-3235-ZJF)

This hydrophobic interaction medium is designed for flow-through purification of antibodies. It uses very large pores exceeding 100 nm to capture high-molecular-weight impurities. Strong phenyl hydrophobicity enables aggregate removal even at relatively low salt concentration. Product antibodies pass through the column while more hydrophobic aggregates are retained. The medium supports sample loading from 50 to 100 g per liter of resin. Product recovery is stated to exceed 95%. Less hydrophobic samples may also be processed in bind-and-elute mode. The medium is applicable to antibodies, aggregate-containing proteins, plasmid DNA, viruses, and other biomacromolecules.

Chromatography mode: Hydrophobic interaction
Ligand: Phenyl
Pore size:>100 nm
Operating mode: Flow-through or bind-and-elute
Recommended loading: 50-100 g/L resin
Product recovery:>95%
Catalog number: 23554
Applications: Antibodies, protein aggregates, plasmid DNA, viruses, and biomacromolecules

Flow-through antibody polishing
Very large pores
Low-salt aggregate removal
Strong phenyl hydrophobicity
High sample loading
Recovery above 95%
Optional bind-and-elute mode
Suitable for large impurities

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