Thiophilic Resin Kit, 20mg Human IgG/ml Resin, 786-266, G-BIOSCIENCES (CAT#: STEM-C-3257-LGZ)

Thiophilic adsorption or thiophilic chromatography is a routine technique for low-cost, simple purification of immunoglobulins. Thiophilic adsorption, first developed by Porath et al. in 1984, is a group-specific, salt-dependent purification technique with pronounced affinity for immunoglobulins and α2-macroglobulin. The principle of thiophilic adsorption is that some proteins in high salt are able to bind to an immobilized ligand containing a sulfone group close to the thioether group. Bound proteins are then eluted upon decreasing salt concentration.

G-Biosciences' Thiophilic Resin binds immunoglobulins, including IgG, IgY, and IgM, from serum, ascitic fluid, or tissue culture supernatant, and then elutes the purified immunoglobulins in a near-neutral aqueous buffer. G-Biosciences' Thiophilic Resin has high binding capacity (~20mg/ml human IgG/ml resin) and broad specificity for immunoglobulin molecules of various species.

Thiophilic adsorption has been used to purify other proteins, including horseradish peroxidase, glutathione peroxidase, lactate dehydrogenase, and allergens.

The Thiophilic Adsorption Kit is designed for the purification of IgG and contains all necessary buffers and columns.

For IgM purification, IgY purification and general protein purification, we recommend our thiophilic resins.

Size: 1

Purified Immunoglobulin G Molecules
High binding capacity (20mg human IgG/ml resin)
Under mild elution conditions of very low salt and near neutral pH
Alternative to Ammonium Sulfate Precipitation Enrichment

Protein G IgG Binding Buffer, 1L, 786-1601, G-BIOSCIENCES (CAT#-STEM-C-3230-LGZ)
Protein G IgG Binding Buffer, 1 Gallon, 786-1602, G-BIOSCIENCES (CAT#-STEM-C-3231-LGZ)
IgG Elution buffer, 100ml, 786-545, G-BIOSCIENCES (CAT#-STEM-C-3232-LGZ)
IgG Elution Buffer, 1L, 786-205, G-BIOSCIENCES (CAT#-STEM-C-3233-LGZ)
IgG Elution Buffer, 1 Gallon, 786-206, G-BIOSCIENCES (CAT#-STEM-C-3234-LGZ)
Gentle IgG Elution Buffer, 100ml, 786-200, G-BIOSCIENCES (CAT#-STEM-C-3235-LGZ)