TALON® Superflow Metal Affinity Resin, 2 x 250 mL, 635669, Takara Bio Inc (CAT#: STEM-C-3828-LGZ)

TALON his-tag purification resins allow you to prepare exceptionally pure his-tag proteins from bacterial, mammalian, yeast, and baculovirus-infected cells under native or denaturing conditions. TALON is a cobalt-laced immobilized metal affinity chromatography (IMAC) resin that binds his-tagged proteins with higher specificity than nickel-laced resins. Therefore, TALON resins provide the highest purity of his-tagged proteins. Furthermore, each cobalt ion binds to the resin at four sites, resulting in low metal ion leakage.

Active core contains the highest purity cobalt

TALON superfluid metal affinity resins are complexed with cobalt ions, making them highly selective for other labeled proteins. The cobalt-containing reactive core of TALON has strict steric requirements—adjacent histidines can only bind if they contain adjacent histidines or are specifically positioned for proteins. Nickel-based resins (such as Ni-NTA) are less steric - these resins have a higher affinity for randomly positioned (i.e. non-His-tagged) histidines. Therefore, TALON resin binds more specifically to his-tagged proteins.
TALON Superflow Metal Affinity Resin is designed for fast and efficient purification of his-tagged proteins at high flow rates and medium pressures (up to 150 psi), ideal for FPLC applications.

Uniform matrix ensures less contamination

Cobalt-based resins have a more uniform structure than nickel-based resins. TALON resins contain negatively charged reactive sites that form three-dimensional pockets. Each pocket contains three carboxyl groups and a nitrogen atom, which together bind a positively charged cobalt ion—an arrangement that allows the cobalt ion to bind to two adjacent histidine residues. In this structure, the cobalt is very tightly bound and cannot be leached from the resin. Nickel-based resins are less homogeneous in structure because nickel ions can form two different coordination complexes: one of them forms three-dimensional pockets similar to TALON ligands, and the other forms planar (flat) structures. In the twisted planar structure, each nickel ion is bound to only two carboxyl groups and one nitrogen atom. This planar structure therefore binds the nickel ions less tightly, allowing them to leach out of the resin. TALON metal affinity resins have a homogeneous matrix that provides high-affinity binding under a variety of purification conditions, ensuring optimal protein purification.

Size: 2 x 250 mL

Has a high affinity for its tagged protein
Ideal for FPLC applications
No protein co-purification
Anti-metal leakage
Performs well under a wide range of purification conditions

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