T-maze Test (CAT#: STEM-AE-0016-WXH)

Tests of Discriminative Learning, Working Memory, and Reference Memory in Animals.

1. Adaptation: Sprinkle 6 food pills in the maze arm to let the rats adapt to the maze.
2. Forced choice training: the animal is forced to choose the food reinforcement arm and complete the feeding.
3. Delayed matching-to-position (DMP).
4. Put the animal back into the cage after one matching training, and then repeat the next matching training.
When the number of correct choices of animals reaches 15/16 for two consecutive days, it is considered to meet the standard and the experiment can be started. If the animal still fails to meet the standard after 30 days of training, it will be eliminated.
5. One day after the animal training reaches the standard, a matching training will be given.
6. In the next two days, 10 matching trainings were given every day, with an interval of 60 seconds between each training, to evaluate the working memory operation of the animals. The number of times the food-fortified arm was entered and the number of times the non-fortified arm was re-entered was recorded. The latter are considered working memory errors. Normal healthy young rats performed accurately almost every time.