Symbiotic Actinomycetes Actinobacteria Probe-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-2525-ZJF)

Employing probe-based real-time PCR, this kit detects Symbiotic Actinomycetes Actinobacteria by amplifying its conserved genetic regions with highly specific primers. Fluorescence signals are emitted during amplification and continuously monitored, allowing for accurate qualitative and quantitative analysis of Symbiotic Actinomycetes Actinobacteria nucleic acids.

Kit Components:
Symbiotic Actinomycetes Actinobacteria Amplification Mix: 50 µL × 1 tube
Symbiotic Actinomycetes Actinobacteria Reaction Mix: 950 µL × 1 tube
Symbiotic Actinomycetes Actinobacteria Positive Control: 50 µL × 1 tube
Symbiotic Actinomycetes Actinobacteria Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: Reagents in the kit are sufficient for the number of tests indicated on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light, avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
Compatible with real-time PCR systems including ABI, MX3000P/3005P, LightCycler, Bio-Rad, and Eppendorf.
Additional required equipment: DNase/RNase-free 1.5 mL centrifuge tubes, 0.2 mL PCR reaction tubes, filter tips (10 µL, 200 µL, 1000 µL), centrifuge, vortex mixer, and pipettes of various capacities.
Sample Requirements:
Depending on circumstances, use intestinal tissue from dead or culled cattle under sterile conditions. For live animals, samples may include whole blood, serum, plasma, feces, vomit, oral/nasopharyngeal/respiratory secretions, throat/nasal/ocular/anal swabs, or viral cultures.
Cross-contamination must be avoided. Samples should be tested promptly after collection or stored at -20°C ± 5°C. For long-term storage, samples should be kept below -70°C.
Sample Storage and Transport:
Short-term: Store at -20°C.
Long-term (up to 6 months): Store below -70°C.
Transport using 2-8°C ice packs. Repeated freeze-thaw cycles are strictly prohibited.

Simplified operation with pre-mixed reagents requiring only DNA input.
Uses conserved region alignment to ensure high specificity.
Detects low-copy targets with high sensitivity due to optimized chemistry.
Contains a positive control for result validation.
Enclosed one-tube protocol limits contamination.
Fast turnaround for time-sensitive applications.
Restricted to laboratory research applications.

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