Study Peptide-Membrane Interactions by Dual polarization interferometry (DPI) (CAT#: STEM-MB-0397-WXH)

Introduction

The common structural features of membrane-active peptides involve the adoption of a stable secondary structure upon binding to the membrane surface. The induction of a cationic amphipathic α-elical structure has been well-characterized and plays an essential role in the membrane perturbing activities of antimicrobial peptides. In contrast, similar structure−activity relationships are less well-studied for membrane-active peptides and proteins that adopt a β-sheet structure upon membrane association, although β-sheet antimicrobial peptides such as the defensins and protegrins have been wellcharacterized. Since these models all involve the binding of peptides to the membrane, the determination of the relative affinity of the peptides for a particular target membrane is central to the delineation of the mechanism of action of these peptides.

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Principle Applications Procedure Materials Notes Related Products

Principle

Dual polarization interferometry (DPI) is an analytical technique that allows the simultaneous determination of thickness, density, and mass of a biological layer on a sensing waveguide surface in real time. DPI focuses laser light into two waveguides. One of these functions as the "sensing" waveguide having an exposed surface while the second one functions to maintain a reference beam. A two-dimensional interference pattern is formed in the far field by combining the light passing through the two waveguides. The DPI technique rotates the polarization of the laser, to alternately excite two polarization modes of the waveguides. Measurement of the interferogram for both polarizations allows both the refractive index and the thickness of the adsorbed layer to be calculated. These measurements can be used to infer conformational information about the molecular interactions taking place, as the molecule size (from the layer thickness) and the fold density (from the RI) change.

Applications

Study Peptide-Membrane Interactions.

Procedure

1. Setting of dual polarization interferometry
2. Preparing the DPI sensor chip
3. Immobilization of target on DPI biosensor
4. Reagent was injected to react
5. Quantitative analysis

Materials

• DPI biosensor
• DPI sensor chip

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