Study on the Interaction Between Proteins and Some Complexon Dyes by Resonance Rayleigh Scattering Method (CAT#: STEM-ST-0027-YJL)

Introduction

A complexing agent containing an amino group (one NH3) and a carboxyl group (one COOH) in the molecule. The reagent molecule contains multiple coordination atoms (O and N), which can form stable chelates with most metal ions, and are often used in complexometric titration. The most commonly used aminocarboxyl complexing agent is ethylenediaminetetraacetic acid (EDTA). Studying the interaction of such dyes with proteins and their effects on RRS spectra can not only expand the types and scope of dyes that react with proteins. Moreover, it is of great significance for clarifying the reactive groups of different dyes and further elucidating the reaction mechanism and the reason for RS enhancement.

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Principle Applications Procedure Materials Notes Related Products

Principle

Resonance Rayleigh scattering (RRS) is similar to Rayleigh scattering in nature. Resonance Rayleigh scattering is a special elastic scattering produced when the wavelength of Rayleigh scattering (RS) is located at or close to its molecular absorption band. The key to generating RRS is: when the scattering is at or close to the absorption band of the scattering molecule, since the electron absorbs the electromagnetic wave at the same frequency as the scattering frequency, the electron strongly absorbs the photon energy due to resonance and re-scatters. Its scattering intensity is several orders of magnitude higher than that of pure Rayleigh scattering, and it no longer obeys the Rayleigh law of I∝λ-4. This absorption-rescattering process is called resonance Rayleigh scattering (RRS).

Applications

Resonance Rayleigh scattering is used to the study of aggregation of chromophores on biological macromolecules and the determination of biological macromolecules such as nucleic acid, proteins and heparin, further, it has been used in the determination of trace amounts of inorganic ions and the cationic surfactant by means of ion association reactions with some dyes. In addition, it has been applied to the study of nanoparticles in liquid and the determination of β-cyclodextrin inclusion constant and the critical micelle concentration of surfactant.

Procedure

1. Sample preparation
2. Measurement by scattering detection instrument
3. Data analysis

Materials

Rayleigh scattering measurement system

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