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Study of Membrane proteins by Linear dichroism (LD) (CAT#: STEM-ST-0111-WXH)

Introduction

The insertion of membrane proteins into a lipid membrane has been monitored using LD, supplying the experimentalist with information about the orientation of the protein relative to the lipid membrane at different time points.




Principle

Linear dichroism (LD) or diattenuation is the difference between absorption of light polarized parallel and polarized perpendicular to an orientation axis. It is the property of a material whose transmittance depends on the orientation of linearly polarized light incident upon it. LD measurements are based on the interaction between matter and light and thus are a form of electromagnetic spectroscopy.

Applications

Study the functionality and structure of molecules.

Materials

• Polarizer
• Absorbance spectrometer