Study of mechanosensing proteins by Magnetic tweezers (MT) (CAT#: STEM-MB-1244-WXH)

Introduction

In tissues, cells need to adapt to their microenvironment by sensing various chemical and physical cues. Recent development in cell biology has demonstrated mechanical force as one of the critical determinants involved in cell migration, cell differentiation, tissue development and maintenance. In tissues, cells adhere to extracellular matrix (ECM) through formation of integrin dependent focal adhesion and to the neighboring cells through cadherin dependent cell–cell adherens junctions. Forces are generated by actomyosin contraction, and propagated in the whole tissue. Force sensing of cells is mediated by a set of mechanosensing proteins located at focal adhesion and cell–cell adherens junctions as well as in the cytoskeleton network.

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Principle Applications Procedure Materials Notes Related Products

Principle

Magnetic tweezers (MT) are scientific instruments for the manipulation and characterization of biomolecules or polymers. These apparatus exert forces and torques to individual molecules or groups of molecules. It can be used to measure the tensile strength or the force generated by molecules.

Applications

Study of mechanical properties of biological macromolecules like DNA or proteins in single-molecule experiments.
Study of the rheology of soft matter.
Study of force-regulated processes in living cells.

Procedure

1.Sample preparation
2.Force Calibration
3.Measurement
4.Analysis

Materials

Magnetic tweezers

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