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Study of histamine-secreting cells by Patch clamp (CAT#: STEM-PET-0036-WXH)

Introduction

Histamine is a chemical immune system releases. It's mainly known for its role in causing allergy symptoms. But it has other important functions, like regulating sleep-wake cycle and cognitive function. Antihistamines are a common medication that can manage histamine levels.<br />It can be found in most of the tissues of the body, but is in higher concentrations in the lungs, skin and the gut. Usually found in basophils and mast cells. Released from mast cells during inflammatory or allergic reactions.




Principle

The patch-clamp technique involves a glass micropipette forming a tight gigaohm seal with the cell membrane. The micropipette contains a wire bathed in an electrolytic solution to conduct ions. To measure single ion channels, a “patch” of membrane is pulled away from the cell after forming a gigaohm seal.

Applications

• Study of ionic currents in individual isolated living cells, tissue sections, or patches of cell membrane.
• Study of excitable cells such as neurons, cardiomyocytes, muscle fibers, and pancreatic beta cells.
• Study of ion channels.

Procedure

1. Fabrication of glass electrodes
2. Measuring glass electrode resistance and compensating offset potential
3. Glass electrode contact to cell membrane and obtain a GΩ seal
4. Acquire and analyse recordings using the appropriate software.

Materials

Patch clamp system