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Structural analysis of Bilirubin by Circular dichroism (CD) (CAT#: STEM-MB-0636-WXH)
Introduction
Bilirubin is a red-orange compound that occurs in the normal catabolic pathway that breaks down heme in vertebrates. This catabolism is a necessary process in the body's clearance of waste products that arise from the destruction of aged or abnormal red blood cells.
Principle
Circular dichroism (CD) is a spectroscopy technique that measures the absorption difference between left and right circularly polarized light. By symmetry, this asymmetric absorption can only occur for asymmetric molecules, meaning chiral molecules.
Applications
Circular dichroism (CD) spectroscopy is a powerful technique that is sensitive to the chirality (handedness) of molecules. It can be used to study absolute stereochemistry, enantiomeric composition, racemization, enantiomeric differentiation, and molecular interactions and conformation.
Procedure
1. Sample preparation
2. Measurement by CD instrument
3. Data analysis
2. Measurement by CD instrument
3. Data analysis
Materials
Circular dichroism (CD) spectrophotometer
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