Streptococcus pyogenes Probe-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-1470-ZJF)

This kit utilizes probe-based real-time fluorescent PCR technology. Specific primers are designed to target highly conserved regions of the Streptococcus pyogenes gene. The system enables in vitro amplification and detection of Streptococcus pyogenes nucleic acids. When the target nucleic acid is present, the PCR reaction proceeds and generates fluorescence signals. These signals are monitored in real time by the instrument to provide both qualitative and quantitative results.

Kit Components:
Streptococcus pyogenes Amplification Mix: 50 µL × 1 tube
Streptococcus pyogenes Reaction Mix: 950 µL × 1 tube
Streptococcus pyogenes Positive Control: 50 µL × 1 tube
Streptococcus pyogenes Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: Reagents in the kit are sufficient for the number of tests indicated on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light, avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
Compatible with real-time PCR systems including ABI, MX3000P/3005P, LightCycler, Bio-Rad, and Eppendorf.
Additional required equipment: DNase/RNase-free 1.5 mL centrifuge tubes, 0.2 mL PCR reaction tubes, filter tips (10 µL, 200 µL, 1000 µL), centrifuge, vortex mixer, and pipettes of various capacities.
Sample Requirements:
Depending on circumstances, use intestinal tissue from dead or culled cattle under sterile conditions. For live animals, samples may include whole blood, serum, plasma, feces, vomit, oral/nasopharyngeal/respiratory secretions, throat/nasal/ocular/anal swabs, or viral cultures.
Cross-contamination must be avoided. Samples should be tested promptly after collection or stored at -20°C ± 5°C. For long-term storage, samples should be kept below -70°C.
Sample Storage and Transport:
Short-term: Store at -20°C.
Long-term (up to 6 months): Store below -70°C.
Transport using 2-8°C ice packs. Repeated freeze-thaw cycles are strictly prohibited.

All reagents pre-prepared; template DNA addition is the only step.
Probes and primers designed for precise recognition of target sequence.
High-sensitivity detection capability built into the system.
Includes positive control for experimental accuracy.
Closed-tube design reduces the chance of contamination.
Accelerated protocol supports time-saving diagnostics.
Only for use in research environments.

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