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Spirochaetoidea and Protozoa pathogenous for man as seen in fixed unstained smears by dark-field illumination or the phase contrast microscope (CAT#: STEM-MIT-0334-LJX)

Introduction

Spirochaeta is a kind of active prokaryotic cell microorganism with spiral shape, which is between bacteria and protozoa in biological position. Spirochaeta is widely distributed in nature and animal body, also exists in human mouth or animal body.




Principle

The main difference between a dark field microscope and an ordinary microscope is the way of illumination. It illuminates the specimen with a strong, narrow, slanted beam without letting the beam enter the objective lens. When no light enters the objective lens, the field of view is dark, so it is called dark field microscope. However, because the particles in the specimen can scatter light after being illuminated by light, when the scattered light enters the objective lens, the scattered light spots of the particles can be seen in the microscope, as if the particles themselves were glowing. This is just as in a dark room, through a small hole in the wall of a strong beam of sunlight, we can see the presence of dust in the light path. This phenomenon, in optics, is known as the Dundar phenomenon. The dark-field microscope is designed according to this principle.

Applications

For observing microorganisms, colloid chemistry, single-celled organisms, and objects with linear structure
Unsuitable for observing stained specimens

Procedure

1. Sample preparation
2. Assembly and adjustment of dark field microscope
3. Observation

Materials

• Sample Type:
Spirochaetoidea and Protozoa pathogenous

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures