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Spectrophotometric pH measurements in rivers, estuaries, and oceans by thymol blue indicator (CAT#: STEM-PPA-0023-LJX)
Introduction
Thymol blue (TB) is one of a suite of indicator dyes appropriate for spectrophotometric determinations of the pH of aqueous solutions. For measurements of seawater pH, meta-cresol purple (mCP) is most often used, but TB is especially well suited for measurements in surface or shallow waters where the pH may exceed the optimal indicating range of mCP (e.g., due to photosynthesis).
Principle
Acid-base indicators produce different colors in aqueous solutions of their specific pH range. The indicator is added to the standard buffer solution, and the resulting color is used as the standard colorimetric tube. The pH value of the water sample can be measured by visual colorimetric comparison with the water sample colorimetric tube with the same indicator.
Applications
For determining the approximate PH value of solutions in food production, wastewater treatment, pharmaceutical and chemical industries
Procedure
1. Add ph indicator to the standard solution, and the solution with different pH will show different colors. Use these solutions as standard colorimetric tubes
2. Add the same ph indicator to the solution to be tested, and compare the color of the solution to be tested with the standard colorimetric tube to determine the approximate ph value of the solution
2. Add the same ph indicator to the solution to be tested, and compare the color of the solution to be tested with the standard colorimetric tube to determine the approximate ph value of the solution
Materials
• Sample Type:
Seawater
Seawater
Notes
After dropping the indicator into the solution, it should be gently shaken to avoid inaccurate detection.
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Wide range of applications
For various laboratory needs
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