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Speciation and analysis of mercury, arsenic, and selenium by Atomic fluorescence spectrometry (AFS) (CAT#: STEM-ST-0137-WXH)

Introduction

Mercury, arsenic and selenium are important elements from the perspective of human and environmental health because of their toxicity and the fact that selenium plays a dual role: as an essential nutrient at low concentrations, or a toxin when in excess.




Principle

Atomic fluorescence spectroscopy (AFS) is the emission of radiation energy in the UV-visible region from gas-phase atoms that have been excited to higher energy levels by absorption of radiant energy. Usually, a flame is used to obtain the atom in a gaseous state. It is a radiative emission process that proceeds from the lowest singlet (S1) to the singlet ground state(S0).

Applications

Atomic Fluorescence Spectrometry (AFS) is an analytical technique that is primarily used to detect and quantify metals. It is an exceptionally sensitive technique that relies on exploiting the distinctive fluorescent spectra of each specific metal.

Procedure

1. Sample preparetion
2. Sample ionization
3. Detection

Materials

• Line Source
• Nebulizer-burner system
• Monochromators/Filters
• Detector, Amplifier, Readout