Silicon Analysis in Biological Specimens by Atomic emission spectroscopy (AES) (CAT#: STEM-ST-0181-WXH)

Introduction

Silicon is the second most abundant element on earth, comprising approximately 28% of the earth's crust. Occurring naturally in a variety of forms, silicon is utilized by plants for rigidity in cell wails and plant stalks. In humans, silicon has been demonstrated to be necessary for growth and bone calcification and may function as a biological cross-linking agent of connective-tissue-based membrane structures.

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Principle Applications Procedure Materials Notes Related Products

Principle

Atomic emission spectroscopy (AES) is a method of chemical analysis that uses the intensity of light emitted from a flame, plasma, arc, or spark at a particular wavelength to determine the quantity of an element in a sample. The wavelength of the atomic spectral line in the emission spectrum gives the identity of the element while the intensity of the emitted light is proportional to the number of atoms of the element. The sample may be excited by various methods.

Applications

Common applications of atomic emission spectroscopy are in the analysis of trace elements in soils, water, metals, biological specimens, clinical specimens, food, physical evidence such as glass, and other solids.

Procedure

1. Prepare sample for analysis
2. Convert solution into aerosol
3. Introduce aerosol into excitation source
4. Atomization and excitation
5. Detection of emission lines

Materials

• ICP (Inductively Coupled Plasma) source
• Atomizer
• Monochromators
• Detectors
• Amplifiers

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