This multimodal chromatography medium is designed for intermediate and polishing purification of viruses and other biomacromolecules. It uses high-flow agarose microspheres with a shell-layer structure. Octylamine ligands provide adsorption interactions in addition to size-based separation. The core-shell architecture captures contaminants while allowing selected target macromolecules to flow through. This combination provides both gel-filtration and adsorption-separation functions. A dynamic ovalbumin capacity above 13 mg/mL indicates useful impurity-binding capability. The medium supports high linear flow with low operating backpressure. Broad pH and chemical stability permit repeated cleaning-in-place. The matrix can be scaled for larger downstream bioprocessing operations.
Specification
Model: Seplife Suncore 700 Appearance: White spherical particles, odorless and tasteless Matrix: High-flow agarose microspheres Ligand: Octylamine Ionic content: 40-85 µmol Cl-/mL Median particle size d50v: 85 µm Dynamic binding capacity:>13.0 mg ovalbumin/mL Operating temperature: 4-30°C Flow performance: 500 cm/h at pressure <0.2 MPa and 20 cm bed height Long-term pH stability: 3-13 Short-term CIP pH stability: 2-14 Chemical compatibility: Common aqueous buffers, 1 mol/L sodium hydroxide, 70% ethanol, 30% isopropanol, and 6 M guanidine hydrochloride Storage: 20% ethanol at 4-30°C