This reversed-phase chromatography medium is designed for separation of small molecules, peptides, low-molecular-weight proteins, and related biomolecules. It uses a porous polystyrene-divinylbenzene polymer matrix. The matrix provides a large surface area and strong physical and chemical stability. Its polymeric construction offers a wider usable pH range than conventional silica media. The 30 µm particles support fast preparative chromatography. High dynamic loading reduces the required packed-bed volume. The medium is compatible with a broad range of aqueous and organic mobile phases. It can be cleaned under strongly acidic or alkaline conditions within the stated limits. The source specification identifies the product as LXMS-30(100), while the supplied product name states LXMS-30(300).
Specification
Model: Seplife® RP LXMS-30(300) Source specification model: Seplife® RP LXMS-30(100) Appearance: White spherical particles Matrix: Polystyrene-divinylbenzene Particle size: 30±3 µm Listed average pore size: 100 Å Wet apparent density: 0.80-0.90 g/mL Water content: 50-80% Recommended flow rate: 100-1000 cm/h Long-term pH stability: 2-12 Short-term CIP pH stability: 1-14 Dynamic loading: 32-40 mg vitamin B12/mL and 30-60 mg IN/mL Maximum pressure: 4 MPa Storage: 20% ethanol at 4-30°C Chemical compatibility: Common aqueous buffers, hydrochloric acid, acetic acid, guanidine hydrochloride, alcohols, acetone, sodium hydroxide, TFA solutions, acetonitrile, isopropanol, and tetrahydrofuran
Features
Broad pH operating range High-flow polymeric matrix Strong organic-solvent compatibility High dynamic loading 30 µm preparative particles Up to 4 MPa pressure Suitable for peptides and small molecules CIP-compatible reversed-phase medium