Seplife RP LXMS-15(300) Reversed-Phase Medium, 15 µm (CAT#: STEM-BC-3117-ZJF)

Highlights

High-resolution 15 µm particles
Large 300 Å pores
Up to 5 MPa pressure

Cat Number: STEM-BC-3117-ZJF

Application: for high-resolution reversed-phase biomolecule separation

Model: Seplife® RP LXMS-15(300)




Description

This reversed-phase chromatography medium is designed for high-resolution purification of small molecules, peptides, low-molecular-weight proteins, and related biomolecules. It uses a porous polystyrene-divinylbenzene polymer matrix. The 15 µm particle size provides greater chromatographic efficiency than larger preparative particles. A 300 Å pore structure supports access by peptides and relatively larger biomolecules. The rigid polymer matrix tolerates high pressure and fast flow. Broad pH stability allows acidic, neutral, and alkaline process conditions. The material is compatible with many aqueous buffers and organic solvents. High dynamic loading supports productive preparative purification. Storage in aqueous ethanol maintains the medium before use.

Specification

Model: Seplife® RP LXMS-15(300)
Appearance: White spherical particles
Matrix: Polystyrene-divinylbenzene
Particle size: 15±2 µm
Average pore size: 300 Å
Wet apparent density: 0.80-0.90 g/mL
Water content: 50-80%
Recommended flow rate: 100-1000 cm/h
Long-term pH stability: 2-12
Short-term CIP pH stability: 1-14
Dynamic loading: 32-40 mg vitamin B12/mL and 30-60 mg IN/mL
Maximum pressure: 5 MPa
Storage: 20% ethanol at 4-30°C
Chemical compatibility: Common aqueous buffers, hydrochloric acid, acetic acid, guanidine hydrochloride, alcohols, acetone, sodium hydroxide, TFA solutions, acetonitrile, isopropanol, and tetrahydrofuran

Features

High-resolution 15 µm particles
Large 300 Å pores
Up to 5 MPa pressure
Broad pH tolerance
Extensive solvent compatibility
High dynamic loading
Suitable for peptides and proteins
Fast preparative operation

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