Seplife LXPM Phenyl 5504M Hydrophobic Interaction Medium, 45-90 µm (CAT#: STEM-BC-3121-ZJF)

This hydrophobic interaction chromatography medium is designed for purification of antibodies, proteins, peptides, nucleic acids, and related biomacromolecules. It uses a polyacrylate matrix with a hydrophilically modified surface. Phenyl groups provide the hydrophobic interaction sites required for separation. Biomolecules adsorb to the medium under high-salt conditions according to their surface hydrophobicity. Reducing the salt concentration elutes molecules in order of increasing hydrophobic interaction strength. The 1000 Å pore structure supports access by large biological molecules. The rigid particles provide high flow, useful binding capacity, and stable chromatographic resolution. The medium can be regenerated with low-ionic-strength buffer and cleaned with sodium hydroxide or ethanol.

Model: Seplife® LXPM Phenyl 5504M
Matrix: Polyacrylate
Appearance: White opaque spherical particles
Functional group: Phenyl
Particle size: 45-90 µm
Pore size: 1000 Å
Dynamic binding capacity: ≥20 mg lysozyme/mL
Flow range: 100-800 cm/h
pH range: 2-12
Maximum pressure: 8 bar
Storage solution: 20% ethanol
Regeneration: Low-ionic-strength buffer
Cleaning: 0.2-0.5 M sodium hydroxide or 50% ethanol
Chemical stability: Resistant to acids, alkalis, methanol, ethanol, toluene, DMSO, DMF, and n-heptane

Phenyl hydrophobic ligand
Mild separation conditions
High chromatographic resolution
1000 Å pore structure
Up to 800 cm/h flow
Broad solvent resistance
Low nonspecific adsorption
Suitable for capture through polishing

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