Seplife LXPM Butyl 5504M Hydrophobic Interaction Medium, 45-90 µm (CAT#: STEM-BC-3122-ZJF)

This hydrophobic interaction medium is designed for purification of antibodies, proteins, peptides, nucleic acids, and other biological macromolecules. It uses a polyacrylate matrix with a hydrophilically modified particle surface. Butyl groups provide moderate hydrophobic interaction sites for biomolecule binding. Proteins adsorb under high-salt conditions according to differences in surface hydrophobicity. Gradual reduction of salt concentration releases molecules according to their interaction strength. The 1000 Å pores facilitate transport of large biological molecules through the particles. The medium combines useful binding capacity with high resolution and stable mechanical properties. It can be regenerated with low-ionic-strength buffer and cleaned with alkaline solution or ethanol.

Model: Seplife® LXPM Butyl 5504M
Matrix: Polyacrylate
Appearance: White opaque spherical particles
Functional group: Butyl
Particle size: 45-90 µm
Pore size: 1000 Å
Dynamic binding capacity: ≥20 mg lysozyme/mL
Flow range: 100-800 cm/h
pH range: 2-12
Maximum pressure: 8 bar
Storage solution: 20% ethanol
Regeneration: Low-ionic-strength buffer
Cleaning: 0.2-0.5 M sodium hydroxide or 50% ethanol
Chemical stability: Resistant to acids, alkalis, methanol, ethanol, toluene, DMSO, DMF, and n-heptane

Butyl hydrophobic interaction
Moderate ligand hydrophobicity
High-resolution protein separation
Large 1000 Å pores
High-flow operation
Broad chemical stability
Regenerable chromatography medium
Suitable for process-scale purification

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