This cell-culture microcarrier is designed for suspension cultivation of anchorage-dependent cells. It uses a dextran-based matrix derived from Seplife G50. Positively charged DEAE groups cover the particle surface and promote cell attachment. The microcarrier is suitable for more than 60 reported cell types. It provides a large effective growth area for high-density cell expansion. The low buoyant density supports suspension and mixing in cell-culture vessels. The particles swell in physiological saline to form the working microcarrier size. Functional testing with Vero cells confirms suitable cell-growth performance relative to the control. The product is intended for vaccine, recombinant protein, and related biological-product manufacturing.
Specification
Model: Seplife LX-MC-Dex1 Appearance: White particles, odorless and tasteless Matrix: Seplife G50 dextran Surface group: Positively charged DEAE Buoyant density in physiological saline: <1.045 g/mL Dry particle size: 60-87 µm for>70% of particles Wet particle size: 145-240 µm Particle size in physiological saline: 145-240 µm Ion-exchange capacity: 1.4-1.6 mmol Cl-/g dry powder Swelling volume: 17-22 mL/g dry powder Loss on drying: <10% Microbial count: <100 CFU/g Heavy-metal content: <5 µg/g Effective culture area: 3500-5500 cm²/g Vero-cell slope ratio versus control:>0.8
Features
Large cell-growth surface Positively charged attachment surface Suitable for more than 60 cell types Low buoyant density Defined swelling performance Verified Vero-cell functionality Supports suspension bioreactor culture Suitable for vaccine and protein production