Seplife 4B Size-Exclusion Medium, 60000-20×10⁶ Da (CAT#: STEM-BC-3177-ZJF)

This traditional agarose gel-filtration medium is designed for samples with substantial molecular-size differences. It uses four-percent crosslinked agarose gel particles. Separation occurs according to the ability of molecules to enter the internal pore network. Larger species are excluded from more pores and therefore elute earlier. Smaller species enter more of the pore volume and elute later. The medium provides low nonspecific adsorption and high product recovery. It can be reused in repeated purification cycles. The product is suitable for applications that do not require very high resolution. It is applicable to proteins, nucleic acids, and peptides in downstream processing.

Model: Seplife 4B
Appearance: White spherical gel, odorless and tasteless
Matrix: 4% crosslinked agarose gel
Globular-protein exclusion range: 60000-20×10⁶ Da
Particle size: 45-165 µm
Pressure-flow range: 70-140 cm/h at 0.15 MPa
Long-term pH stability: 4-9
Short-term CIP pH stability: 3-11
Chemical compatibility: Common gel-filtration solutions, 8 mol/L urea, and 6 mol/L guanidine hydrochloride
Heat resistance: 121°C in pH 7 water for 30 min

Traditional size-exclusion medium
Very broad exclusion range
Low nonspecific adsorption
High product recovery
Reusable agarose particles
Denaturant-compatible operation
Steam-treatment tolerance
Suitable for large biomolecules

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