Sensitive detection of tumour cells in effusions by Fluorescence in situ hybridisation (FISH) (CAT#: STEM-MB-1206-WXH)

Name: Sensitive detection of tumour cells in effusions by Fluorescence in situ hybridisation (FISH)
SKU: STEM-MB-1206-WXH
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Introduction

Effusion fluids in cancer patients can arise in the pleural, pericardial, and peritoneal space. Pathologic accumulation of such fluid is driven by different forces, among which are ‘paraneoplastic’ effects triggered directly or indirectly by tumour cells.
Diagnosis of malignant cells in effusions is important for staging procedures and resulting therapeutic decisions. Cytodiagnostics in effusions is sometimes difficult since reactive mesothelial cells can mimic malignant cells.

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Principle Applications Procedure Materials

Principle

FISH uses fluorescent probes with complementary base sequences to locate the presence or absence of specific portions of DNA on chromosomes. The probe and target DNA must be denatured with heat or chemicals to break hydrogen bonds in the DNA and to allow hybridisation to occur once the two samples are mixed. The fluorescent probes form new hydrogen bonds with their complementary base pairs on the DNA, and these can then be detected via microscopy.

Applications

Detect and localize the presence or absence of specific DNA sequences on chromosomes.
Detect and localize specific RNA targets (mRNA, lncRNA and miRNA) in cells, circulating tumor cells, and tissue samples.

Procedure

1. Sample preparation
2. Co-denaturation and hybridization
3. Probe detection
4. Wash off of unbound probe
5. Analysis by flow cytometer/fluorescence microscopy