Seahorse XFe96 Analyzer (CAT#: AM-LE-0324-Y)

Highlights

Cat Number: AM-LE-0324-Y

Application: • Test to detect mitochondrial function • Determination of ATP produced by simultaneous quantitative analysis of glycolysis and oxidative phosphorylation • A metabolic phenotype can be generated within an hour • Determining and measuring the rate of glycolysis in living cells • Detect the dependence of cellular energy production on mitochondrial substrates • Analysis of three-dimensional samples such as cell spheres and islets

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Description Specification Features Related Products

Description

The Seahorse XFe96 Analyzer is manufactured by Agilent Technologies and can be used to determine the OCR and ECAR of live cells in 96-well plates to understand the cellular metabolic function of cultured cells and in vitro samples.

Specification

Number of analysis holes: 92
Microchamber volume: 2 µL
Plate specifications: 96-well microplate
Controller: Windows 10 computer combined with a touch screen display with complete test design, control and analysis functions.
Software: Design and run analysis templates on Wave Controller or Wave Desktop.
Main advantages:
Maximum experimental flexibility
Highest flux
Verify under hypoxic conditions
Verification at a detection temperature of 16–42 °C (requires a temperature below room temperature of 12–20 °C)

Features

• Analyze only 5000 cells per well in a custom 96-well plate The cell number requirements vary according to cell type
• Has a precision temperature-controlled heating tray that can maintain 16-42 °C (12-20 °C above room temperature), so it is compatible with a variety of samples
• Wave software can control the instrument and perform real-time rate measurement to provide results on the day of measurement
• The integrated system can report metabolic rate in minutes without sample extraction or labeling
• Real-time detection of the response of substrates, inhibitors and other compounds, dosing through the 4 dosing port system and automatic mixing, while maintaining physiological temperature (37 °C)
• The 96-well plate format can test a variety of conditions in one analysis, which is ideal for dose response studies and compound screening

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