rProtein G Seplife 4FF Antibody Affinity Medium, 30 mg Human IgG/mL (CAT#: STEM-BC-3144-ZJF)

This Protein G affinity medium is designed for purification of antibodies from different species. It uses a four-percent high-flow agarose matrix coupled with recombinant Protein G. The ligand selectively binds immunoglobulins and compatible Fc fragments. It can process cell-culture supernatants and cell extracts. Multipoint ligand coupling reduces ligand loss during repeated operation. The medium is suitable for both laboratory-scale and industrial-scale IgG purification. High-flow performance allows relatively rapid processing of large sample volumes. Broad chemical compatibility supports exposure to selected denaturants, detergents, acids, and alcohols.

Model: rProtein G Seplife 4FF
Appearance: Spherical gel, odorless and tasteless
Matrix: Seplife 4FF
Ligand: Recombinant Protein G
Shape: Spherical
Particle size: 45-165 µm
Pressure-flow performance:>220 cm/h at 0.1 MPa
Binding capacity: 30 mg human IgG/mL
Ligand density: 2 mg/mL
Long-term pH stability: 3-9
Short-term CIP pH stability: 2-10
Chemical compatibility at below 37°C for 7 days: 6 M guanidine hydrochloride, 70% ethanol, 20 mM sodium phosphate, 1% SDS at pH 7.0, and 1 M acetic acid at pH 2.0
Short-term compatibility: 0.1 M hydrochloric acid at pH 1.0 and 8 M urea at pH 10.5

Recombinant Protein G ligand
30 mg/mL human IgG capacity
Broad species antibody binding
Fc-fragment purification
Multipoint ligand coupling
High-flow agarose matrix
Laboratory-to-industrial scalability
Broad reagent compatibility

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