Recombinant Rat TNF α Protein, Seafrom (CAT#: STEM-CT-0040-ZJF)

The Recombinant Rat TNF α Protein manufactured by Seafrom is suitable for pro-inflammatory effects and immune response regulation.
Tumor Necrosis Factor-Alpha (TNF-α) is a pleiotropic pro-inflammatory cytokine secreted by various cell types, including adipocytes, activated monocytes, macrophages, B cells, T cells, and fibroblasts. It belongs to the TNF ligand family and signals through two receptors, TNFR1 and TNFR2. TNF-α exhibits cytotoxicity against various tumor cells and plays a crucial role in mediating immune responses to bacterial infections. It is involved in septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, and diabetes induction. Both human and mouse TNF-α show significant cross-species reactivity.
TNF-α exists in two forms: a type II transmembrane protein and a mature soluble protein. Proteolytic cleavage of the transmembrane TNF-α yields a biologically active 17 kDa soluble form, which can form non-covalently linked homotrimers in solution.
The lyophilized product can be stored at 2-8°C for 24 months. For reconstitution, it can be dissolved in injectable water, saline solution, culture medium, or PBS. After dissolution, it should be aliquoted, with a recommended storage period of 6 months at -20°C and 12 months at -80°C. When diluting, ensure a final concentration of no less than 100 μg/mL and avoid repeated freeze-thaw cycles.

Expression Host: HEK293 Cells
Synonyms: Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin
Protein Sequence: DNA sequence encodes rat TNF-α (NP_036807.1) with a His tag at the C-terminus, predicted molecular weight of 18.9 kD
Purity: >90%, analyzed by SDS-PAGE
Endotoxin Level: <1 EU/μg
Biological Activity: Lysis capability determined using mouse L929 cells in the presence of actinomycin D, ED ≤ 0.05 ng/mL, corresponding specific activity is ≥2x10^7 IU/mg
Purification Method: Chromatographic purification
Appearance: White loose body

Stable Cell Library
Large-Scale Expression
High Activity
Batch Stability
Gmp-Quality Control
Ensured Product Quality

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