Recombinant Murine VEGF165 Protein, Seafrom (CAT#: STEM-CT-0061-ZJF)

The Recombinant Murine VEGF165 Protein manufactured by Seafrom is suitable for promoting angiogenesis and vascular permeability.
VEGF, or Vascular Endothelial Growth Factor, is a potent cell growth and angiogenesis factor. It stimulates endothelial cell proliferation and survival, promoting angiogenesis and vascular permeability. VEGF is expressed in vascularized tissues and plays a critical role in normal and pathological angiogenesis. Extensive evidence suggests that VEGF plays a crucial role in inducing tumor metastasis and ocular neovascular syndromes. VEGF is expressed through FMS-like tyrosine kinase (Flt-1), KDR gene product (mouse homolog is FLK-1 gene product), and FLT 4 gene product.
The lyophilized formulation can be stored at 2-8°C for 24 months. If further dilution is required, it can be reconstituted with injection-grade water, physiological saline, culture medium, or PBS. Once reconstituted, it should be aliquoted, and the aliquots should be stored at -20°C for 6 months or at -80°C for 12 months, with a post-dilution concentration not less than 100 μg/mL, while avoiding repeated freeze-thaw cycles.

Expression Host: HEK293 Cells
Synonyms: Vascular Endothelial Growth Factor, Follicle Stimulating Hormone, Glioma-derived Endothelial Cell Mitogen, Vascular Permeability Factor
Protein Sequence: DNA sequence encodes recombinant mouse VEGF165 protein (NP_001273986.1) with a His tag at the C-terminus, contains 170 amino acids, predicted molecular weight of 20.1 kD
Purity: >90%, analyzed by SDS-PAGE
Endotoxin Level: <1 EU/μg
Biological Activity: Dose-dependent stimulation of human umbilical vein endothelial cell (HUVEC) proliferation, effective concentration range of 1.0-5.0 ng/mL
Purification Method: Chromatographic purification
Appearance: White loose body

Stable Cell Library
Large-Scale Expression
High Activity
Batch Stability
Gmp-Quality Control
Ensured Product Quality

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