Recombinant Human VEGFR2/KDR (mFc Tag), Yisheng (CAT#: STEM-CT-0210-ZJF)

The Recombinant Human VEGFR2/KDR (mFc Tag) manufactured by Yisheng is suitable for studying VEGFR2-mediated signaling in angiogenesis and cancer.
VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1), and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). VEGFR2 cDNA encodes a 1356 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGFR2 is composed of a 745 aa residue extracellular domain, a 25 aa residue transmembrane domain, and a 567 aa residue cytoplasmic domain. In contrast to VEGFR1, which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

Synonyms: CD309, KDR, VEGFR, VEGFR2, VEGFR-21, FLK1, KRD1, Ly73
Uniprot No.: P35968-1
Source: Recombinant Human VEGF R2/KDR Protein is expressed from HEK293 Cells with mFc tag at the C-terminal. It contains Ala20-Glu764.
Molecular Weight: Approximately 110 kDa. Due to glycosylation, the protein migrates to 150-200 kDa based on Tris-Bis PAGE result.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: > 95% as determined by SDS-PAGE and HPLC
Activity: ELISA Data: Immobilized Human VEGF165 1 μg/mL (100 μL/Well) on the plate. Dose response curve for Human VEGF R2, mFc Tag with the EC50 of 36.1 ng/mL determined by ELISA.
Endotoxin: < 1.0 EU per 1μg of the protein by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Shipped with ice pack.
Stored at -20℃ to -80℃ for 1 year.
2-7 days, 2 to 8 ℃ under sterile conditions after reconstitution.
3 months, -20 ℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.
For safety and health, please wear lab coats and disposable gloves for operation.

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