Recombinant Human FOLR1 (His-Avi Tag), Yisheng (CAT#: STEM-CT-0212-ZJF)

The Recombinant Human FOLR1 (His-Avi Tag) manufactured by Yisheng is suitable for targeting FOLR1-positive cells in cell therapy.
Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37-42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Mature FOLR1 is an N-glycosylated protein anchored to the cell surface by a GPI linkage. Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1. FOLR1 is predominantly expressed on epithelial cells and is dramatically upregulated on many carcinomas. It is critically required during early embryogenesis, as shown in knockout mice, which die in utero with gross morphological defects.

Synonyms: FR-alpha, FR alpha, FR α, FOLR, FBP, Folbp1, KB cells FBP, MOv18, Fbp1, Folate receptor alpha
Uniprot No.: P15328
Source: Recombinant Human FOLR1 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Arg25-Met233.
Molecular Weight: Approximately 27.5 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: > 95% as determined by SDS-PAGE and HPLC
Activity: ELISA Data: Immobilized Human FOLR1, His Tag at 0.5 μg/mL (100 μL/Well) on plate. Dose response curve for Anti-FOLR1 Antibody, hFc Tag with the EC50 of 11.9 ng/mL determined by ELISA.
Endotoxin: < 1.0 EU per 1μg of the protein by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Shipped with ice pack.
Stored at -20℃ to -80℃ for 1 year.
2-7 days, 2 to 8 ℃ under sterile conditions after reconstitution.
3 months, -20 ℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.
For safety and health, please wear lab coats and disposable gloves for operation.

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