Recombinant Human Fibronectin (FN)Protein, Seafrom (CAT#: STEM-CT-0057-ZJF)

The Recombinant Human Fibronectin (FN)Protein manufactured by Seafrom is suitable for promoting cell adhesion and supporting cell migration.
Recombinant human fibronectin fragments, which include the cell-binding domain, heparin-binding domain, and the CS1 site, significantly enhance retrovirus-mediated gene transfer into mammalian cells. This effect is likely due to the co-localization of retroviral particles and target cells on rhFN CH-296 chimeric molecules through direct binding of retroviral particles and regions containing heparin-binding domain II and cell adhesion sites VLA-4 and/or VLA-5. According to the research results of Hannenberg and others, using this coating method, a transient culture of retrovirus-containing supernatant without cells typically achieves 50% to 75% gene transduction.
The lyophilized formulation can be stored at -20°C for 24 months. If further dilution is required, it can be done with injection-grade water, physiological saline, culture medium, or PBS. Once diluted, it can be stored at -20°C for 6 months or at -80°C for 12 months, with a post-dilution concentration not less than 100 μg/mL, while avoiding repeated freeze-thaw cycles.

Expression Host: E. coli
Synonyms: Cold-insoluble globulin
Protein Sequence: Fragment of human Fibronectin (P02751) DNA-encoded, with a His tag at the N-terminus, predicted molecular weight of 64.7 kD
Purity: >90%, analyzed by SDS-PAGE
Endotoxin Level: <0.1 EU/μg
Biological Activity: Promotes hiPS cell adhesion with an ED50 of ≤3.2 μg/mL
Purification Method: Chromatographic purification
Appearance: Colorless transparent liquid

Stable Cell Library
Large-Scale Expression
High Activity
Batch Stability
Gmp-Quality Control
Ensured Product Quality

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