Quantitative Fluorescent PCR (QF-PCR) (CAT#: STEM-MB-0186-WXH)

Introduction

Quantitative Fluorescence PCR adds fluorescently labeled probes on the basis of conventional PCR to achieve its quantitative function. Fluorescence quantitative PCR realizes the quantitative and qualitative analysis of the initial template by detecting the fluorescence signal of each cycle product in the PCR amplification reaction in real time. According to the different markers used, fluorescent quantitative PCR can be divided into chimeric fluorescent dye method and fluorescent probe method. Compared with ordinary PCR, fluorescent quantitative PCR has the following advantages:<br />1. Blocking reaction, no need for PCR post-processing<br />2. Strong specificity and high sensitivity<br />3. Using logarithmic phase analysis, discarding endpoint data, accurate quantification<br />4. Wide quantitative range, up to 10 orders of magnitude<br />5. The online real-time detection of the instrument, the result is intuitive and avoids human judgment<br />6. It can realize double inspection or multiple inspection in one tube<br />7. Safe operation, shorten time and improve efficiency