Quantitative Analysis of Taxol Injection by Near Infrared (NIR) Spectroscopy (CAT#: STEM-ST-1480-CJ)
Introduction
Taxol is a cancer medication that interferes with the growth and spread of cancer cells in the body. Taxol is used to treat breast cancer, ovarian cancer, and lung cancer. It is also used to treat AIDS-related Kaposi's sarcoma.
Near-infrared spectroscopy (NIRS) is a method that uses light between the visible and infrared wavelength ranges (approximately 800 – 2500 nm) and measures the absorption of light as a function of wavelength. The near-infrared light that interacts with the sample has specific wavelengths that are absorbed based on certain chemical bonds (e.g. C-H, O-H, S-H, and N-H) being present in the sample.
Applications
Biomedical; Biopharmaceuticals
Procedure
1. Prepare samples. 2. Place the sample in the Near Infrared Spectroscopy. 2. Analyze data and identify samples.
1. NIR is non-destructive, and requires little or no sample preparation. 2. NIR reflectance spectra can be used to quickly determine a material’s properties without altering the sample. 3. NIR can depending on wavelength penetrate up to 20 mm of a sample which makes NIR more effective for solid samples. 4. FTIR spectroscopy is, in principle, very similar to Near Infrared (NIR) spectroscopy, but works at longer wavelengths where the chemical information from the samples is more specific.