PROTEINDEX™ rProtein A Agarose 4 Fast Flow, 5 mL, 11-0203-005, Marvelgent biosciences (CAT#: STEM-C-3685-LGZ)

PROTEINDEX™ rProtein A Sepharose 4 Fast Flow is an affinity chromatography medium designed for simple one-step purification of immunoglobulins, especially of the IgG isotype, and fusion proteins containing the Fc region.

Protein A is a bacterial cell wall protein isolated from Staphylococcus aureus that binds to the Fc region of most immunoglobulins through interaction with the heavy chain. Native protein A contains five high-affinity IgG-binding domains and many domains with unknown functions.

Recombinant protein A is a genetically engineered protein that contains only 5 high-affinity IgG binding domains. Non-essential domains were removed to minimize non-specific binding. Recombinant protein A is produced in E. coli and it binds immunoglobulins with essentially the same affinity as native protein A. Protein A can have a high affinity with the Fc region of IgG of various species such as human and rabbit.

Through the proprietary ProteIndex™ technology, the immobilization of recombinant protein A on 4% highly cross-linked agarose resin is very stable, resulting in high yields in laboratory preparations and scale-up applications.

Size: 5 mL
Ligand: Recombinant protein A with Ig binding domains
Binding capacity: >40 mg human IgG/mL medium
Matrix Cross-linked: 4% agarose supplied as a 50% slurry
Particle size: 45 μm - 165 μm
Maximum pressure: 0.3 MPa (3 bar)
Chemical stability: All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.
Recommended flow rate: Gravity flow and medium pressure, 30 - 300 cm/hour
pH stability: Long term: pH 2 - 9; Short term: pH 2 - 11
Storage temperature: 2 - 8°C
Storage buffer: 20% ethanol

Large volume, > 40 mg human IgG / mL of settled resins
High selectivity, high purity
Mechanically stable matrix support for spin (up to 1000g) and automation applications
Ligand leakage is minimal and reusable
Versatile, ready-to-use convenient format

Chromatography Columns, 1 Adjustable End, 5-7bar, 5mm ID, 900mm Length, 7bar, GB-5-900-1TE, G-BIOSCIENCES (CAT#-STEM-C-3370-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 5mm ID, 1000mm Length, 7bar, GB-5-1000-1TE, G-BIOSCIENCES (CAT#-STEM-C-3371-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 8mm ID, 100mm Length, 7bar, GB-8-100-1TE, G-BIOSCIENCES (CAT#-STEM-C-3372-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 8mm ID, 200mm Length, 7bar, GB-8-200-1TE, G-BIOSCIENCES (CAT#-STEM-C-3373-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 8mm ID, 300mm Length, 7bar, GB-8-300-1TE, G-BIOSCIENCES (CAT#-STEM-C-3374-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 8mm ID, 400mm Length, 7bar, GB-8-400-1TE, G-BIOSCIENCES (CAT#-STEM-C-3375-LGZ)