PROTEINDEX™ Recombinant Protein A Prepacked Gravity Flow Columns, 1 mL, Pack Of 5 Columns, Particle Size 45 μm - 165 μm, 18-0268, Marvelgent biosciences (CAT#: STEM-C-3698-LGZ)

PROTEINDEX™ Recombinant Protein A Sepharose Gravity Flow Column is a ready-to-use affinity column for the purification of immunoglobulins, especially of the IgG isotype, and fusion proteins containing the Fc region.

Protein A is a bacterial cell wall protein isolated from Staphylococcus aureus that binds to the Fc region of most immunoglobulins through interaction with the heavy chain. Native protein A contains five high-affinity IgG-binding domains and many domains with unknown functions.

Recombinant protein A is a genetically engineered protein that contains only 5 high-affinity IgG binding domains. Non-essential domains were removed to minimize non-specific binding. Recombinant protein A is produced in E. coli and it binds immunoglobulins with essentially the same affinity as native protein A. Protein A can have a high affinity with the Fc region of IgG of various species such as human and rabbit.

Through the proprietary ProteIndex™ technology, the immobilization of recombinant protein A on 4% agarose resin is very stable and optimized for high yield in laboratory formulation and scale-up applications.

Each gravity column contains 1ml of recombinant protein A sepharose resin and is individually wrapped.

Product package size: 5 columns
Column volume: 1 mL settled Protein A resins
Ligand: Recombinant protein A produced in E.coli
Binding capacity: >40 mg human IgG
Matrix: 4% agarose
Particle size: 45 μm - 165 μm
Maximum pressure: 0.1 MPa (1 bar)
Chemical stability: All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.
Recommended flow rate: Gravity flow, 100 - 300 cm/hour
pH stability: Long term: pH 3 - 9; Short term: pH 2 - 10
Storage temperature: 2 - 8°C
Storage buffer: 20% ethanol
Column material: polypropylene (body); Low density polyethylene (top snap cap and bottom tip cap)
Frit: Polyethylene, with an average pore size of 30 μm

Chromatography Columns, 1 Adjustable End, 5-7bar, 10mm ID, 200mm Length, 7bar, GB-10-200-1TE, G-BIOSCIENCES (CAT#-STEM-C-3383-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 10mm ID, 300mm Length, 7bar, GB-10-300-1TE, G-BIOSCIENCES (CAT#-STEM-C-3384-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 10mm ID, 400mm Length, 7bar, GB-10-400-1TE, G-BIOSCIENCES (CAT#-STEM-C-3385-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 10mm ID, 500mm Length, 7bar, GB-10-500-1TE, G-BIOSCIENCES (CAT#-STEM-C-3386-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 10mm ID, 600mm Length, 7bar, GB-10-600-1TE, G-BIOSCIENCES (CAT#-STEM-C-3387-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 10mm ID, 700mm Length, 7bar, GB-10-700-1TE, G-BIOSCIENCES (CAT#-STEM-C-3388-LGZ)