PI3K-Akt Pathway Assay (CAT#: STEM-MB-0352-WXH)

Akt regulates cell growth through its effects on the TSC1/TSC2 complex and mTORC signaling. Akt contributes to cell proliferation via phosphorylation of the CDK inhibitors p21 and p27. Akt is a major mediator of cell survival through direct inhibition of pro-apoptotic proteins like Bad or inhibition of pro-apoptotic signals generated by transcription factors like FoxO. Akt is critically involved in the regulation of metabolism through activation of AS160 and PFKFB2. In addition, Akt has been shown to regulate proteins involved in neuronal function including the GABA receptor, ataxin-1 and huntingtin proteins. Akt contributes to cell migration and invasion via phosphorylation of palladin and vimentin. Akt also regulates NF-κB signaling by phosphorylating IKKα and Tpl2. Due to the critical role of Akt/PKB in regulating diverse cellular functions it is an important therapeutic target for the treatment of human disease.

To study the effect of each virus on PI3K-Akt signaling pathway
To study the regulation mechanism of PI3K-Akt signal pathway in disease
To study the effects of drugs or therapies on PI3K-Akt signaling pathways

• Luminex Multiplex Assay
• Enzyme-linked immunosorbent assay (ELISA)
• Flow cytometry (FACS analysis) technology

Detectable, targets:, RTKs, SH2, PDK1, PIP2, AKT, PKB, mTORC2, mTOR, PRRS, Sin1, Rictor, TSC1, TSC2, Rheb, mTORC1, GBL, S6K, 4E-BP1, ELF4E, CREB, p53, CREB, MDM2, Bax, BIM, Bcl-2, FoxO1, GSK3, GS, BAD, p27, p21, Ras, p85, p110