Phenyl Seplife HP Hydrophobic Interaction Medium, 24-44 µm (CAT#: STEM-BC-3145-ZJF)

This high-performance hydrophobic interaction medium is designed for fine separation of biological macromolecules. It uses a high-resolution agarose matrix functionalized with phenyl ligands. The phenyl groups interact with hydrophobic regions on proteins and related molecules. Binding occurs under elevated salt conditions and elution occurs as salt concentration decreases. The 24-44 µm particles provide higher chromatographic resolution than larger process beads. A protein adsorption capacity of 24 mg HSA/mL supports preparative polishing. Spherical particles provide uniform packing and reproducible separation. Broad chemical and pH stability support repeated purification and cleaning cycles.

Model: Phenyl Seplife HP
Appearance: White spherical gel, odorless and tasteless
Matrix: Seplife 6FF
Ligand: Phenyl
Shape: Spherical
Particle size: 24-44 µm
Maximum flow rate: 150 cm/h
Pressure resistance: 0.3 MPa
Operating temperature: 4-40°C
Ligand density: 25 µmol phenyl/mL medium
Protein adsorption capacity: 24 mg HSA/mL medium
Long-term pH stability: 3-13
Short-term CIP pH stability: 2-14
Chemical compatibility: 1 mol/L sodium hydroxide, 70% ethanol, 30% isopropanol, 0.5% SDS, 10% ethylene glycol, 6 mol/L guanidine hydrochloride, 8 mol/L urea, and 3 mol/L ammonium sulfate

High-resolution 24-44 µm particles
Strong phenyl interaction
24 mg/mL HSA capacity
Designed for polishing
Broad chemical stability
Wide CIP pH range
Uniform spherical gel
Suitable for biomacromolecules

STEM-BC-0412-ZJF
STEM-BC-3074-ZJF
STEM-BC-3075-ZJF
STEM-BC-3078-ZJF
STEM-BC-3081-ZJF