Phenyl Large Scale Hydrophobic Interaction Medium,>600 cm/h (CAT#: STEM-BC-3134-ZJF)

This hydrophobic interaction medium is designed for process-scale purification of biological macromolecules. It uses high-flow agarose microspheres functionalized with phenyl ligands. The ligand provides strong hydrophobic interaction with exposed nonpolar regions on biomolecules. Binding is promoted under high-salt conditions and elution occurs as ionic strength decreases. The approximately 75 µm particles balance binding capacity and flow performance. Dynamic HSA capacity above 27 mg/mL supports productive downstream processing. The medium operates above 600 cm/h at the stated pressure and bed height. Broad pH and chemical stability support repeated regeneration and cleaning. Its scalable format is suitable for biopharmaceutical capture, intermediate purification, and polishing.

Model: Phenyl Large Scale
Appearance: White spherical gel, odorless and tasteless
Type: Strong hydrophobic interaction medium
Matrix: Large Scale microspheres
Ligand: Phenyl
Median particle size d50v: Approximately 75 µm
Dynamic binding capacity QB10:>27 mg HSA/mL
Ligand density:>27 µmol/mL
Operating temperature: 4-30°C
Flow performance:>600 cm/h at 0.3 MPa differential pressure and 20 cm bed height
Long-term pH stability: 3-13
Short-term CIP pH stability: 2-14
Chemical compatibility: 1 mol/L sodium hydroxide, 70% ethanol, 30% isopropanol, 0.5% SDS, 10% ethylene glycol, 6 mol/L guanidine hydrochloride, 8 mol/L urea, and 3 mol/L ammonium sulfate

High-flow process-scale medium
Strong phenyl interaction
>27 mg/mL HSA capacity
Low operating backpressure
Broad chemical stability
Wide CIP pH tolerance
Scalable agarose particles
Suitable for downstream bioprocessing

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