Pearl™ Monoclonal IgG Purification Kit, 1L Serum/ 0.2L Ascites, 786-802, G-BIOSCIENCES (CAT#: STEM-C-3256-LGZ)

Pearl™ Monoclonal IgG Purification Kit provides rapid purification of antibodies from cell culture supernatants and ascites fluid. Pearl™ IgG Purification Resin binds high-abundance, non-IgG proteins (i.e. albumin) and allows IgG molecules to pass through physiological buffers. IgG molecules can be stored or used in downstream applications without further cleanup, such as ammonium sulfate precipitation.

The Pearl™ Monoclonal IgG Purification Kit can be used to purify antibodies directly from cell culture supernatants containing less than 10% FBS, or from ascites fluid treated with the provided ascites pretreatment.

Pearl™ Monoclonal IgG Purification Kit can purify IgG from ~1L cell culture supernatant or 200ml ascitic fluid.

Complete IgG purification kits are also available, in two formats:

A spin-on format suitable for rapid, small-scale purification of IgG. The kit provides 3ml Pearl™ IgG Purification Resin, IgG Isolation Buffer and 20 spin columns. Suitable for purification of IgG up to 25mg.
A larger format kit provides 25ml Pearl™ IgG Purification Resin and IgG Isolation Buffer, suitable for IgG isolation from ~100ml serum (~200mg IgG).

Size: 1L Serum/ 0.2L Ascites

Isolation of monoclonal antibodies from ascites fluid or cell culture supernatant
Provides ascitic fluid pretreatment reagents for optimal IgG purification
For 1L cell culture supernatant or 0.2L ascitic fluid

Protein G IgG Binding Buffer, 100ml, 786-1600, G-BIOSCIENCES (CAT#-STEM-C-3229-LGZ)
Protein G IgG Binding Buffer, 1L, 786-1601, G-BIOSCIENCES (CAT#-STEM-C-3230-LGZ)
Protein G IgG Binding Buffer, 1 Gallon, 786-1602, G-BIOSCIENCES (CAT#-STEM-C-3231-LGZ)
IgG Elution buffer, 100ml, 786-545, G-BIOSCIENCES (CAT#-STEM-C-3232-LGZ)
IgG Elution Buffer, 1L, 786-205, G-BIOSCIENCES (CAT#-STEM-C-3233-LGZ)
IgG Elution Buffer, 1 Gallon, 786-206, G-BIOSCIENCES (CAT#-STEM-C-3234-LGZ)