Observation of ultrastructure of cilia by transmission electron microscopy technology (CAT#: STEM-MIT-0004-LJX)

Introduction

Cilia is a long, swinging protrusion extending from the free surface of a cell, thicker and longer than microvilli, which can be seen under a light microscope. A cell can have hundreds of cilia. Ciliae length is about 5-10 μm, thickness approximately 0.2 μm. There is a dense particle at the root, called the basalbody. <br />The cilia have the ability to oscillate rhythmically in a certain direction. Many cilia coordinate and sway together, pushing secretions and granular substances adhering to the epithelial surface towards a certain direction. For example, most of the lumen surface of the respiratory tract is ciliated epithelium, which, due to the directional oscillation of cilia, can expel inhaled dust and bacteria.

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Principle Applications Procedure Materials Notes Related Products

Principle

Transmission electron microscopy (TEM) is to project the accelerated and concentrated electron beam onto a very thin sample, and the electron collides with the atoms in the sample and changes the direction, thus generating the stereo scattering Angle. The size of the scattering Angle is related to the density and thickness of the sample, so the image can be formed with different shades. The image can be enlarged, focused and displayed on imaging devices such as fluorescent screens, film and photosensitive coupling components. The resolution of transmission electron microscope is much higher than that of optical microscope, can reach 0.1~0.3nm, magnification of tens of thousands to millions of times. Therefore, transmission electron microscopy can be used to observe the fine structure of the sample.

Applications

Microscopic imaging in materials science or biology.

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Cilia

Notes

Pay attention to air humidity
Voltage needs to be stabilized

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