Nocardial Clostridial Bacillus Probe-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-2302-ZJF)

This kit offers a robust and sensitive solution for the detection of Nocardial Clostridial Bacillus using advanced real-time PCR technology. With specially designed primers targeting conserved sequences of the Nocardial Clostridial Bacillus gene, the kit delivers high specificity and real-time fluorescence monitoring for accurate qualitative and quantitative analysis.

Kit Components:
Nocardial Clostridial Bacillus Amplification Mix: 50 µL × 1 tube
Nocardial Clostridial Bacillus Reaction Mix: 950 µL × 1 tube
Nocardial Clostridial Bacillus Positive Control: 50 µL × 1 tube
Nocardial Clostridial Bacillus Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: Reagents in the kit are sufficient for the number of tests indicated on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light, avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
Compatible with real-time PCR systems including ABI, MX3000P/3005P, LightCycler, Bio-Rad, and Eppendorf.
Additional required equipment: DNase/RNase-free 1.5 mL centrifuge tubes, 0.2 mL PCR reaction tubes, filter tips (10 µL, 200 µL, 1000 µL), centrifuge, vortex mixer, and pipettes of various capacities.
Sample Requirements:
Depending on circumstances, use intestinal tissue from dead or culled cattle under sterile conditions. For live animals, samples may include whole blood, serum, plasma, feces, vomit, oral/nasopharyngeal/respiratory secretions, throat/nasal/ocular/anal swabs, or viral cultures.
Cross-contamination must be avoided. Samples should be tested promptly after collection or stored at -20°C ± 5°C. For long-term storage, samples should be kept below -70°C.
Sample Storage and Transport:
Short-term: Store at -20°C.
Long-term (up to 6 months): Store below -70°C.
Transport using 2-8°C ice packs. Repeated freeze-thaw cycles are strictly prohibited.

Ready-to-use format; users only need to provide sample DNA.
Primers and probes are designed from conserved regions for high specificity.
Optimized oligo sequences deliver excellent sensitivity.
Positive control is included to help identify false-negative results.
Closed-tube, single-step workflow minimizes contamination risk.
Fast detection with minimal processing time.
For research use only.

STEM-SLSR-2300-ZJF
STEM-SLSR-2301-ZJF
STEM-SLSR-2303-ZJF
STEM-SLSR-2304-ZJF