Nanosize Particle Analysis by Dynamic light scattering (DLS) by Dynamic light scattering (DLS) (CAT#: STEM-MB-0548-WXH)

Introduction

Dynamic light scattering (DLS) is used for measuring average particle diameter and particle diameter distribution of nano-sized particles dispersed in liquid. The parameters are important characteristics mainly for drug delivery system (DDS) formulations, such as solid in oil (S/O) formulations, liposome formulations, suspension formulations, and emulsion formulations.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Dynamic Light Scattering (DLS) is an established and precise measurement technique for characterizing particle sizes in suspensions and emulsions. It is based on the Brownian motion of particles - this states that smaller particles move faster, while larger ones move slower in a liquid. The light scattered by particles contains information on the diffusion speed and thus on the size distribution.
The Dynamic Light Scattering (DLS) technique measures motion optically by recording the scattered light signal at a fixed angle. The particles are illuminated with a monochromatic, coherent light source (laser) and the light scattered by the particles is recorded.

Applications

DLS is used to characterize the size of various particles including proteins, polymers, micelles, Protein cages and virus-like particles, vesicles, carbohydrates, nanoparticles, biological cells, and gels.

Procedure

1. Sample preparation
2. Measurement by DLS instrument
3. Data analysis

Materials

• Dynamic Light Scattering (DLS) instruments (Photon Correlation Spectroscopy or Quasi-Elastic Light Scattering instruments)
• Dynamic Light Scattering Detector

Other recommended products

Characterize the size of micelles by Dynamic light scattering (DLS) (CAT#: STEM-MB-0555-WXH)
Measurement of casein micelle size by Dynamic light scattering (DLS) by Dynamic light scattering (DLS) (CAT#: STEM-MB-0547-WXH)
Homogeneity studies of Thyroglobulin by Dynamic light scattering (DLS) (CAT#: STEM-MB-0533-WXH)
Homogeneity studies of Aprotinin v (CAT#: STEM-MB-0538-WXH)
Tracing protein aggregates by Dynamic light scattering (DLS) (CAT#: STEM-MB-0549-WXH)
Characterization of PKR–RNA complex by Dynamic light scattering (DLS) (CAT#: STEM-MB-0542-WXH)
Characterize the size of vesicles by Dynamic light scattering (DLS) (CAT#: STEM-MB-0557-WXH)
Assessement of the molecular integrity and stability of monoclonal antibodies by Dynamic light scattering (DLS) (CAT#: STEM-MB-0552-WXH)