Mycoplasma penetrans Probe-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-1746-ZJF)

Designed for the detection of Mycoplasma penetrans, this kit applies probe-based real-time PCR technology. It uses primers targeting conserved gene sequences of Mycoplasma penetrans, enabling efficient amplification and fluorescent signal generation. Real-time monitoring of fluorescence ensures reliable qualitative and quantitative evaluation of the target nucleic acid.

Kit Components:
Mycoplasma penetrans Amplification Mix: 50 µL × 1 tube
Mycoplasma penetrans Reaction Mix: 950 µL × 1 tube
Mycoplasma penetrans Positive Control: 50 µL × 1 tube
Mycoplasma penetrans Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: Reagents in the kit are sufficient for the number of tests indicated on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light, avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
Compatible with real-time PCR systems including ABI, MX3000P/3005P, LightCycler, Bio-Rad, and Eppendorf.
Additional required equipment: DNase/RNase-free 1.5 mL centrifuge tubes, 0.2 mL PCR reaction tubes, filter tips (10 µL, 200 µL, 1000 µL), centrifuge, vortex mixer, and pipettes of various capacities.
Sample Requirements:
Depending on circumstances, use intestinal tissue from dead or culled cattle under sterile conditions. For live animals, samples may include whole blood, serum, plasma, feces, vomit, oral/nasopharyngeal/respiratory secretions, throat/nasal/ocular/anal swabs, or viral cultures.
Cross-contamination must be avoided. Samples should be tested promptly after collection or stored at -20°C ± 5°C. For long-term storage, samples should be kept below -70°C.
Sample Storage and Transport:
Short-term: Store at -20°C.
Long-term (up to 6 months): Store below -70°C.
Transport using 2-8°C ice packs. Repeated freeze-thaw cycles are strictly prohibited.

Just add DNA—kit is fully prepared for immediate use.
Primer and probe sequences selected for highly specific binding.
Maximum sensitivity supported by optimized assay design.
Positive control makes troubleshooting and verification easier.
One-tube closed operation limits contamination sources.
Ideal for quick and efficient analysis.
For investigational use only.

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