Mouse Mitochondrial DNA Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-0044-ZJF)

This kit utilizes SYBR Green dye-based real-time fluorescent PCR technology. Specific primers are designed to target highly conserved regions of the Mouse Mitochondrial DNA gene. In the presence of the target nucleic acid template, PCR amplification occurs, generating fluorescence signals. The fluorescence intensity is monitored in real time, enabling qualitative and quantitative analysis of Mouse Mitochondrial DNA with high sensitivity and specificity.

Kit Components:
Mouse Mitochondrial DNA Amplification Mix: 50 µL × 1 tube
Mouse Mitochondrial DNA Reaction Mix: 950 µL × 1 tube
Mouse Mitochondrial DNA Positive Control: 50 µL × 1 tube
Mouse Mitochondrial DNA Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Ready-to-use kit requiring only sample DNA; the entire detection process (per sample) takes just 2.0 hours.
Primers are designed based on conserved regions of mouse mitochondrial DNA to specifically amplify target sequences without cross-reacting with unrelated DNA.
Based on dye-based fluorescence qPCR, offering higher sensitivity than conventional PCR.
Closed one-tube operation minimizes the risk of cross-contamination.
Includes positive reference standard to support accurate result interpretation.
For research use only; sufficient for 50 fluorescence qPCR reactions using a 2 μL reaction system.

STEM-SLSR-0042-ZJF
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STEM-SLSR-0045-ZJF
STEM-SLSR-0046-ZJF