Molecular mass determination of proteins by Static light scattering (SLS) (CAT#: STEM-MB-0559-WXH)

Introduction

Protein molecular weight is a key parameter to confirm, as the primary structure is the most foundational level of protein structure. Developing a robust understanding of the unmodified protein molecular weight can assist in initial assessments of the biomolecule’s functionality. This could include: Binding and transportation of small molecular species Enzyme catalysis Gene regulation Immunological responses Metabolic regulation

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Principle Applications Procedure Materials Notes Related Products

Principle

Static light scattering is a technique in physical chemistry that measures the intensity of the scattered light to obtain the average molecular weight Mw of a macromolecule like a polymer or a protein in solution. Measurement of the scattering intensity at many angles allows calculation of the root mean square radius, also called the radius of gyration Rg. By measuring the scattering intensity for many samples of various concentrations, the second virial coefficient, A2, can be calculated.

Applications

The main applications of static light scattering is molecular mass determination of macromolecules, such as proteins and polymers, as it is possible to measure the molecular mass of proteins without any assumption about their shape.

Procedure

1. Sample preparation
2. Measurement by SLS instrument
3. Data analysis

Materials

• Right-Angle Light Scattering (RALS) Detector
• Low-Angle Light Scattering (LALS) Detector
• Hybrid RALS/LALS Detector
• Multi-Angle Light Scattering (MALS) Detector

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