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Measurement of the Average Mass of Proteins Adsorbed to a Nanoparticle by Suspended Microchannel Resonator (SMR) (CAT#: STEM-PC-0127-CJ)

Introduction

The use of nanoparticles for biomedical applications has been a major area of study in the last decade and continues to be a rapidly growing field of research. Nanoparticles are rigorously researched in the field of drug delivery and imaging not only because of their size, shape, or high surface to volume ratio, but also because they are looked at as platforms that offer versatile possibilities for modification with functional moieties ranging from small chemical groups to large macromolecules.




Principle

The SMR uses the principle that the resonating frequency of an oscillating cantilever (visualize a diving board in motion) is proportional to its mass. The SMR is made up of a cantilever containing a tiny fluidics channel surrounded by a vacuum. When a single cell flows through the channel inside the cantilever, the resonant frequency of the cantilever changes in proportion to the mass of the cell.

Applications

Biochemistry; Biomedical

Procedure

1. Sample preparation.
2. The sample solution flows through the microchannel in the resonant cantilever, and mechanical resonance occurs to obtain data.
3. Analyse the data.

Materials

• Sample: Particle-based APIs (e.g., VLPs, liposomes, polymeric nanoparticles), Virus Particle, Protein Formulations
• Equipment: Suspended Microchannel Resonator (SMR)

Notes

1. The SMR is a microelectromechanical systems (MEMS) device that can weigh individual cells to a precision better than one part in 1,000.
2. Using a suspended microchannel resonator, researchers can measure the relative positions of tiny particles as they flow through a fluidic channel.