Madurella spp. Probe-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-1823-ZJF)

Employing probe-based real-time PCR, this kit detects Madurella spp. by amplifying its conserved genetic regions with highly specific primers. Fluorescence signals are emitted during amplification and continuously monitored, allowing for accurate qualitative and quantitative analysis of Madurella spp. nucleic acids.

Kit Components:
Madurella spp. Amplification Mix: 50 µL × 1 tube
Madurella spp. Reaction Mix: 950 µL × 1 tube
Madurella spp. Positive Control: 50 µL × 1 tube
Madurella spp. Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: Reagents in the kit are sufficient for the number of tests indicated on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light, avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
Compatible with real-time PCR systems including ABI, MX3000P/3005P, LightCycler, Bio-Rad, and Eppendorf.
Additional required equipment: DNase/RNase-free 1.5 mL centrifuge tubes, 0.2 mL PCR reaction tubes, filter tips (10 µL, 200 µL, 1000 µL), centrifuge, vortex mixer, and pipettes of various capacities.
Sample Requirements:
Depending on circumstances, use intestinal tissue from dead or culled cattle under sterile conditions. For live animals, samples may include whole blood, serum, plasma, feces, vomit, oral/nasopharyngeal/respiratory secretions, throat/nasal/ocular/anal swabs, or viral cultures.
Cross-contamination must be avoided. Samples should be tested promptly after collection or stored at -20°C ± 5°C. For long-term storage, samples should be kept below -70°C.
Sample Storage and Transport:
Short-term: Store at -20°C.
Long-term (up to 6 months): Store below -70°C.
Transport using 2-8°C ice packs. Repeated freeze-thaw cycles are strictly prohibited.

Pre-configured reagents streamline setup—just add template DNA.
High specificity ensured by targeting conserved genetic regions.
Enhanced sensitivity achieved through refined primer/probe optimization.
Includes a positive reference sample for accurate result interpretation.
One-tube qPCR minimizes exposure and cross-contamination.
Rapid detection within a short time frame.
Not for clinical or diagnostic use—research only.

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