M-CSF Detection (CAT#: STEM-MB-0266-WXH)

M-CSF is an N-glycosylated secreted protein. Human M-CSF has a total length of 192 amino acids, and both have similar inverted α helices. M-CSF widely expresses and regulates the development, survival, and function of monocyte cell lines, such as macrophages and dendritic cells. Although macrophages and other monocyte-phagocytic cell lines are also regulated by other growth factors, the most important regulator is M-CSF. M-CSF affects various cellular functions of monocyte cell lines by binding to its receptors.
After CSF-1R binds to CSF-1, its conformation changes to form a dimer, and the tyrosine kinase activity is activated. And tyrosine at positions 699,708,723,809, and 969 undergo phosphorylation in CSF-1R. 699 Tyrosine binds to growth hormone receptor binding protein (Grb2). Phosphorylation of tyrosine at position 708 is associated with activation of STAT1, and tyrosine at position 723 is associated with 3-phosphoryl inositol kinase (PI3 -K) to transmit signals.

M-CSF mainly regulates the very early and late responses of cells in the G1 phase, and is a growth factor necessary for cell proliferation and differentiation.
M-CSF has the effect of stimulating the proliferation and differentiation of granulocytes and enhancing the function of mature granulocytes.
M-CSF plays an important role in the process of non-specific cellular immunity.
M-CSF regulates osteoclast differentiation.

1. Process samples.
2. M-CSF detection (qPCR, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry).
3. Analysis results.

Sample Types-Blood, serum, etc.